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Goat anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)
Catalog Number: GRP12245
Product Overview
| Product Name | Goat anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG) |
|---|---|
| Catalog Number | GRP12245 |
| Species/Host | Goat |
| Reactivity | Rat |
| Predicted Reactivity | Rat IgG Heavy and Light chains (H&L) of all Rat immunoglobulins |
| Tested applications | ELISA, WB, IHC |
| Immunogen | Purified Rat IgG, whole molecule |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Purified goat IgG |
Application Notes
| Dilution Range | 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB) |
|---|---|
| Application Notes | Additional Information: No reactivity is observed to non-immunoglobulin rat serum. Antibody has been absorbed against mouse IgG and no reactivity is observed to human or mouse IgG based on immunoelectrophoresis. HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Background: Goat anti-rat IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily. |
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