Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, Unconjugated

Product Overview

Product Name	Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, Unconjugated
Catalog Number	GRP12698
Species/Host	Goat
Reactivity	Rabbit
Predicted Reactivity	Rabbit IgG Heavy and Light chains (H&L)
Tested applications	ELISA
Immunogen	Purified Rabbit IgG, whole molecule

Product Properties

Form/Appearance	Lyophilized
Storage	Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note	For research use only.
Clonality	Polyclonal
Purity	Purified goat IgG, F(ab)'2 fragment

Application Notes

Dilution Range	The optimal working dilution should be determined by the investigator.
Application Notes	Additional Information: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis. Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Purity is ≥ 90 % based on SDS-PAGE. May contain small amounts of intact IgG. Background: Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody which binds to rabbit IgG in immunological assays. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

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