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Goat anti-Rabbit IgG (H&L), DyLight® 488 conjugated
Catalog Number: GRP12256
Product Overview
| Product Name | Goat anti-Rabbit IgG (H&L), DyLight® 488 conjugated |
|---|---|
| Catalog Number | GRP12256 |
| Species/Host | Goat |
| Reactivity | Rabbit |
| Predicted Reactivity | Rabbit IgG Heavy and Light chains (H&L) |
| Tested applications | ICC, IF, IHC |
| Immunogen | Purified Rabbit IgG, whole molecule |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified goat IgG |
Application Notes
| Dilution Range | 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF) |
|---|---|
| Application Notes | Additional Information: Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE. Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight® 488 has a maximum absorbance at 493 nm; Emax = 518 nm. Background: Goat anti-rabbit IgG, DyLight® 488 Conjugate is a secondary antibody conjugated to DyLight® 488, which binds to all rabbit IgGs in immunological assays.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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