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Goat anti-mouse IgM (µ chain), HRP conjugated, min. cross-reactivity to human IgG/serum

Catalog Number: GRP12749

Product Overview
Product Name Goat anti-mouse IgM (µ chain), HRP conjugated, min. cross-reactivity to human IgG/serum
Catalog Number GRP12749
Species/Host Goat
Product Properties
Form/Appearance Lyophilized
Storage Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note For research use only.
Clonality Polyclonal
Purity Affinity purified goat IgG
Application Notes
Dilution Range 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Application Notes Additional Information: This antibody reacts with the heavy chains on mouse IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins or to human IgG or serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase. Background: Goat anti-mouse IgM, µ chain is a HRP conjugated secondary antibody which binds to mouse IgM, µ chain in immunological assays. It has been specially adsorbed against human IgG/serum. Antibodies are affinity purified using solid phase mouse IgM. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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