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Goat anti-Mouse IgG (H&L), TRITC conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum
Catalog Number: GRP12521
Product Overview
| Product Name | Goat anti-Mouse IgG (H&L), TRITC conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum |
|---|---|
| Catalog Number | GRP12521 |
| Species/Host | Goat |
| Immunogen | Purified mouse IgG (H&L) AAA51107 |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified IgG |
Application Notes
| Dilution Range | The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications |
|---|---|
| Application Notes | Additional Information: Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit. Background: Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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