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Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity to human IgG/serum
Catalog Number: GRP12410
Product Overview
| Product Name | Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity to human IgG/serum |
|---|---|
| Catalog Number | GRP12410 |
| Species/Host | Goat |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified IgG, F(ab)'2 fragment |
Application Notes
| Dilution Range | 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB) |
|---|---|
| Application Notes | Additional Information: This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase. Background: Goat anti-mouse IgG (H&L), HRP conjugated, is a F(ab)'2 fragment, of a secondary antibody which binds to mouse IgG in immunological assays. Antibodies are adsorbed against human IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG. Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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