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Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum
Catalog Number: GRP12678
Product Overview
| Product Name | Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum |
|---|---|
| Catalog Number | GRP12678 |
| Species/Host | Goat |
| Reactivity | Human |
| Predicted Reactivity | Human IgG Fc (two Heavy chains with constant domains) |
| Tested applications | ELISA, WB, IHC |
| Immunogen | Purified human IgG |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified goat IgG |
Application Notes
| Dilution Range | The optimal working dilution should be determined by the investigator. |
|---|---|
| Application Notes | Additional Information: This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunglobulins or non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins or IgG F(ab)' fragment based on immunoelectrophoresis.The antibody ahs been absorbed against solid phase normal bovine, mouse and rabbit serum in addition to mouse and rabbit IgG. HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase Background: Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays. Reconstitution: For reconstitution add 0.55 ml of sterile water |
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