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Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum
Catalog Number: GRP12658
Product Overview
| Product Name | Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum |
|---|---|
| Catalog Number | GRP12658 |
| Species/Host | Goat |
| Reactivity | Human |
| Predicted Reactivity | Human IgG (H&L), F(ab)'2 fragment |
| Tested applications | ELISA, WB, IHC |
| Immunogen | purified human IgG (H&L) |
Product Properties
| Form/Appearance | Liquid |
|---|---|
| Storage | Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified goat IgG |
Application Notes
| Dilution Range | The optimal working dilution should be determined by the investigator. |
|---|---|
| Application Notes | Additional Information: This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis. HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity ≥90 % based on SDS-PAGE. May contain small amounts of intact IgG Background: Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays. |
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