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Goat anti-Chicken IgY (H&L), DyLight® 550 conjugated
Catalog Number: GRP12780
Product Overview
| Product Name | Goat anti-Chicken IgY (H&L), DyLight® 550 conjugated |
|---|---|
| Catalog Number | GRP12780 |
| Species/Host | Goat |
| Reactivity | Chicken |
| Predicted Reactivity | Chicken IgY Heavy and Light chains (H&L) |
| Tested applications | ICC, IHC |
| Immunogen | purified chicken IgY, whole molecule |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified goat IgG |
Application Notes
| Dilution Range | 1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC) |
|---|---|
| Application Notes | Additional Information: Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis. Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight® 550 (Ex = 550 nm; Em = 576 nm). Background: Goat anti-chicken IgY, DyLight® 550 Conjugate is a secondary antibody conjugated to DyLight® 550, which binds to hen immunoglobulins in immunological assays. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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