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Goat anti-Adenovirus, hexon, Polyclonal Antibody, HRP
Catalog Number: GRP823
Product Overview
Product Name | Goat anti-Adenovirus, hexon, Polyclonal Antibody, HRP |
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Catalog Number | GRP823 |
Species/Host | Goat |
Predicted Reactivity | Does not crossreact with Para 1-3, Infl. A & B, RSV, hMPV & Rhinovirus (type 16) |
Conjugation | HRP |
Tested applications | ELISA, IF, WB |
Immunogen | Gradient purified, disrupted Adenovirus type 5 virions. |
Product Properties
Note | For research use only. |
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Isotype | IgG |
Clonality | Polyclonal |
Application Notes
Dilution Range | > 1: 5,000 by ELISA; > 1:8,000 in IFA; > 1:1,000 in western blot immunoassay |
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Application Notes | GRP823 pairs as a reporter with capture monoclonal antibody GRP827 in ELISA for hexon. This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods developed by the manufacturer. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3. This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/ml) and preserved with 0.002% thimerosal. This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for for enzyme immunoassays. Recommended short term ((<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze. Unit size is 1.0 ml. NOTE: sodium azide is a potent inhibitor of peroxidase ! |
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