Goat anti-Adenovirus, hexon, Polyclonal Antibody, FITC

Catalog Number: GRP822

Product Overview

Product Name	Goat anti-Adenovirus, hexon, Polyclonal Antibody, FITC
Catalog Number	GRP822
Species/Host	Goat
Predicted Reactivity	Does not crossreact with Para 1-3, Infl. A & B, RSV, hMPV & Rhinovirus (type 16)
Conjugation	FITC
Tested applications	ELISA, IF, WB
Immunogen	Gradient purified, disrupted Adenovirus type 5 virions.

Product Properties

Note	For research use only.
Isotype	IgG
Clonality	Polyclonal

Application Notes

Dilution Range	> 1: 5,000 by ELISA; > 1:8,000 in IFA; > 1:1,000 in western blot immunoassay
Application Notes	This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/ml) as a stabilizer and 0.1% sodium azide preservative. Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. Manufacturer also recommends use of its COUNTERSTAIN/BLOCKING DILUENT (Cat.#GRP1082) and its MOUNTING MEDIUM (Cat.#GRP1081) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing Manufacturers enzyme-antibody conjugate specific to FITC (Cat.# GRP986). Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze. Immunoglobulin concentration is 4-5 mg/ml. Unit size is 1.0 ml. NOTE: sodium azide is a potent inhibitor of peroxidase !

Dilution Range

> 1: 5,000 by ELISA; > 1:8,000 in IFA; > 1:1,000 in western blot immunoassay

Application Notes

This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/ml) as a stabilizer and 0.1% sodium azide preservative. Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. Manufacturer also recommends use of its COUNTERSTAIN/BLOCKING DILUENT (Cat.#GRP1082) and its MOUNTING MEDIUM (Cat.#GRP1081) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing Manufacturers enzyme-antibody conjugate specific to FITC (Cat.# GRP986). Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze. Immunoglobulin concentration is 4-5 mg/ml. Unit size is 1.0 ml. NOTE: sodium azide is a potent inhibitor of peroxidase !

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