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Product Overview
| Product Name | GNB1 antibody |
|---|---|
| Catalog Number | GRP142 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, IP, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 3.05 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P62873 |
| Entrez | 2782 |
Product Description
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:1000-1:10000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1(GRP594) antibody at 1:500 dilution.
Sample (50 μg of whole cell lysate) A: mouse brain 10% SDS PAGE GRP594 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GNB1 antibody detects GNB1 protein at cell membrane in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. GNB1 antibody (GRP594) diluted at 1:1000.
GNB1 antibody detects GNB1 protein at cell membrane in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GNB1 antibody (GRP594) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GRP594) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GNB1 protein stained by GNB1 antibody (GRP594) diluted at 1:500.Red: FIS1, a mitochondria marker, st
Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 ?g of GNB1 antibody (GRP594).Western blot analysis was performed using GNB1 antibody (GRP594).EasyBlot anti-Rabbit IgG was used as a secondary reagent.
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