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Product Overview
| Product Name | Glyoxalase I antibody [N1C3] |
|---|---|
| Catalog Number | GRP40 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q04760 |
| Entrez | 2739 |
Product Description
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
![Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] (GRP](https://www.grp-ak.de/media/catalog/product/g/l/glyoxalase-i-antibody-n1c3_grp492_if_1_2.jpg)
![Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I antibody [N1C3] (GRP492) diluted at 1:500.Antigen Retrieval: Citrate](https://www.grp-ak.de/media/catalog/product/g/l/glyoxalase-i-antibody-n1c3_grp492_ihc-p_1_2.jpg)
Sample (50 μg of whole cell lysate) A: Mouse brain 12% SDS PAGE GRP492 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] (GRP
Glyoxalase I antibody detects Glyoxalase I protein by Western blot analysis.A. 30 ?g A431 whole cell lysate/extract B. 30 ?g HeLa whole cell lysate/extract C. 30 ?g HepG2 whole cell lysate/extract12 % SDS-PAGEGlyoxalase I antibody (GRP492) dilution: 1:100
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I antibody [N1C3] (GRP492) diluted at 1:500.Antigen Retrieval: Citrate
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