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Glyoxalase I antibody [N1C3]

Catalog Number: GRP40

Product Overview
Product Name Glyoxalase I antibody [N1C3]
Catalog Number GRP40
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 1 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID Q04760
Entrez 2739
Product Description

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000
Validation Images
Sample (50 μg of whole cell lysate) A: Mouse brain 12% SDS PAGE GRP492 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] (GRP
Glyoxalase I antibody detects Glyoxalase I protein by Western blot analysis.A. 30 ?g A431 whole cell lysate/extract B. 30 ?g HeLa whole cell lysate/extract C. 30 ?g HepG2 whole cell lysate/extract12 % SDS-PAGEGlyoxalase I antibody (GRP492) dilution: 1:100
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I antibody [N1C3] (GRP492) diluted at 1:500.Antigen Retrieval: Citrate
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