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Product Overview
| Product Name | GBA antibody [C1C3] |
|---|---|
| Catalog Number | GRP101 |
| Species/Host | Rabbit |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GBA. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.84 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P04062 |
| Entrez | 2629 |
Product Description
This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
![MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GRP553) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](https://www.grp-ak.de/media/catalog/product/g/b/gba-antibody-c1c3_grp553_wb_2_2.jpg)
MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBA antibody [C1C3] (GRP553) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human lung cancer, using GBA(GRP553) antibody at 1:100 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using GBA(GRP553) antibody at 1:100 dilution.
Sample(30 μg of whole cell lysate)A:Hep G2(GRP553)7.5% SDS PAGE diluted at 1:500The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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