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Product Overview
| Product Name | Fumarate hydratase antibody |
|---|---|
| Catalog Number | GRP43 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.61 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P07954 |
| Entrez | 2271 |
Product Description
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (GRP495) antibody at 1:500 dilution.
Fumarate hydratase antibody detects FH protein by western blot analysis. A. 50 μg mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody (GRP495) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the prim
Fumarate hydratase antibody detects Fumarate hydratase protein by western blot analysis.A.50 μg rat liver lysate/extract 10% SDS-PAGEFumarate hydratase antibody (GRP495) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect t
Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarate hydratase stained by Fumarate hydratase antibody (GRP495) diluted at 1:50
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody (GRP495) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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