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FOXO3A antibody [C3], C-term

Catalog Number: GRP5

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Datasheet
Product Overview
Product Name FOXO3A antibody [C3], C-term
Catalog Number GRP5
Species/Host Rabbit
Reactivity Human, Mouse, Rat, Squirrel
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, IP, WB
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXO3A. The exact sequence is proprietary.
Alternative Names (click to expand) forkhead box O3 | AF6q21 | FKHRL1 | FKHRL1P2 | FOXO2 | FOXO3A
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.94 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID O43524
Entrez 2309
Product Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GRP457 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (50 μg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GRP457 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using FOXO3A(GRP457) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using FOXO3A(GRP457) antibody at 1:500 dilution.
FOXO3A antibody immunoprecipitates FOXO3A protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. Control with 2 ?g of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 ?g of FOXO3A antibody (GRP457) 5% SDS-PAGE The im
FOXO3A antibody immunoprecipitates FOXO3A protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. Control with 2 ?g of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 ?g of FOXO3A antibody (GRP457) 5% SDS-PAGE The im
FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:1000.Blue: Hoech
FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:1000.Blue: Hoech
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GRP457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (GRP457) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buff
FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buff
FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buffer,
FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buffer,
FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buffer, p
FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buffer, p
FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 m
FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 m
FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 mi
FOXO3A antibody [C3], C-term detects FOXO3A protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.FOXO3A stained by FOXO3A antibody [C3], C-term (GRP457) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 mi
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