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Product Overview
| Product Name | FOXA1 antibody |
|---|---|
| Catalog Number | GRP6 |
| Species/Host | Rabbit |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | ChIP, ICC, IF, IHC-P, IP, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXA1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P55317 |
| Entrez | 3169 |
Product Description
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500 |
|---|
Validation Images
FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. FOXA1 antibody (GRP458) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXA1(GRP458) antibody at 1:1000 dilution.
FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. FOXA1 antibody (GRP458) dilution: 1:500.
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ?g of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 ?g whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 ?g of pre-immune rabbit IgG C : Immunoprecipitat
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