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FOXA1 antibody

Catalog Number: GRP6

Product Overview
Product Name FOXA1 antibody
Catalog Number GRP6
Species/Host Rabbit
Reactivity Human
Conjugation Unconjugated
Tested applications ChIP, ICC, IF, IHC-P, IP, WB
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXA1. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P55317
Entrez 3169
Product Description

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
FOXA1 antibody detects FOXA1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. FOXA1 antibody (GRP458) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FOXA1(GRP458) antibody at 1:1000 dilution.
FOXA1 antibody detects FOXA1 protein at nucleus on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. FOXA1 antibody (GRP458) dilution: 1:500.
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ?g of either control rabbit IgG or anti-FOXA1 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.
FOXA1 antibody immunoprecipitates FOXA1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 40 ?g whole cell lysate/extract of FOXA1 protein expressing HepG2 cells B : Control with 2.5 ?g of pre-immune rabbit IgG C : Immunoprecipitat
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