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F4/80 antibody [C2C3], C-term

Catalog Number: GRP109

Product Overview
Product Name F4/80 antibody [C2C3], C-term
Catalog Number GRP109
Species/Host Rabbit
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Tested applications IHC-P, WB
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of Human F4/80. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.23 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID Q14246
Entrez 2015
Product Description

This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,IHC-P: 1:100-1:1000
Validation Images
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using EMR1(GRP561) antibody at 1:100 dilution.
EMR1 antibody [C2C3], C-term detects EMR1 protein by western blot analysis.A. 50 μg rat liver lysate/extract7.5% SDS-PAGEEMR1 antibody [C2C3], C-term (GRP561) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with F4/80 antibody [C2C3], C-term (GRP561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with F4/80 antibody [C2C3], C-term (GRP561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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