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Product Overview
Product Name | F4/80 antibody [C2C3], C-term |
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Catalog Number | GRP109 |
Species/Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Conjugation | Unconjugated |
Tested applications | IHC-P, WB |
Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of Human F4/80. The exact sequence is proprietary. |
Alternative Names | (click to expand) |
Product Properties
Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
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Concentration | 0.23 mg/ml |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
Note | For research use only. |
Isotype | IgG |
Clonality | Polyclonal |
Purity | Purified by antigen-affinity chromatography. |
Uniprot ID | Q14246 |
Entrez | 2015 |
Product Description
This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. [provided by RefSeq]
Application Notes
Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000 |
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Validation Images
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using EMR1(GRP561) antibody at 1:100 dilution.
EMR1 antibody [C2C3], C-term detects EMR1 protein by western blot analysis.A. 50 μg rat liver lysate/extract7.5% SDS-PAGEEMR1 antibody [C2C3], C-term (GRP561) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with F4/80 antibody [C2C3], C-term (GRP561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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