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ExoS - Exoenzyme S

Catalog Number: GRP12190

Product Overview
Product Name ExoS - Exoenzyme S
Catalog Number GRP12190
Species/Host Chicken
Reactivity Bacteria
Predicted Reactivity Pseudomonas aeruginosa
Tested applications WB
Immunogen amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
Product Properties
Form/Appearance Liquid in PBS pH 8.0, 0.02% sodium azide
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Note For research use only.
Clonality Polyclonal
Purity Total IgY
MW 48 kDa
Uniprot ID Q51449 , PA3841
Application Notes
Dilution Range 1 : 5000 (WB)
Application Notes Background: Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.
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