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Product Overview
| Product Name | eNOS (phospho Ser1177) antibody |
|---|---|
| Catalog Number | GRP73 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, WB |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human eNOS (phospho Ser1177). The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 0.74 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P29474 |
| Entrez | 4846 |
Product Description
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GRP525) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GRP525) diluted at 1:500.
Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GRP525) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detec
Untreated (–) and treated (+) THP-1 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GRP525) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect t
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GRP525) diluted at 1:500.Antige
eNOS (phospho Ser1177) antibody detects eNOS (phospho Ser1177) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.eNOS (phospho Ser1177) stained by eNOS (phospho Ser1177) antibody (GRP525) diluted at 1:500.Antigen
Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with eNOS (phospho Ser1177) antibody (GRP525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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