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EGFRv3 Polyclonal Antibody

Catalog Number: GRP482

Product Overview
Product Name EGFRv3 Polyclonal Antibody
Catalog Number GRP482
Species/Host Rabbit
Reactivity Human
Conjugation Unconjugated
Tested applications IHC-P, WB
Immunogen KLH conjugated synthetic peptide derived from human EGFRvIII (public_immunogen_range: 1-50/1081)
Alternative Names
Product Properties
Form/Appearance Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Concentration 1ug/ul
Storage Store at -20°C for 12 months.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by Protein A.
Uniprot ID Q59FL8
Entrez 1956
Product Description

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

Application Notes
Dilution Range WB: 1:300-1000, IHC-P: 1:200-400
Validation Images
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti-EGFRvIII Polyclonal Antibody, Unconjugated (GRP482) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A549 Cells lysates probed with EGFRvIII Polyclonal Antibody, unconjugated (GRP482) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (EGFRvIII) polyclonal Antibody, Unconjugated (GRP482) 1:100, 90 minutes at 37°C; fo
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