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Donkey anti-Sheep IgG (H&L), DyLight® 650 conjugated, min. cross-reactivity to human or rabbit IgG
Catalog Number: GRP12908
Product Overview
| Product Name | Donkey anti-Sheep IgG (H&L), DyLight® 650 conjugated, min. cross-reactivity to human or rabbit IgG |
|---|---|
| Catalog Number | GRP12908 |
| Species/Host | Donkey |
| Immunogen | Purified Sheep IgG, whole molecule |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified donkey IgG |
Application Notes
| Dilution Range | 1 : 20-1 : 2000 for most applications |
|---|---|
| Application Notes | Additional Information: Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulinsNo reactivity is observed to: non-immunoglobulin sheep serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. Background: Donkey anti-Sheep IgG (H&L) - DyLight®650 Conjugated, min. cross-reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight® 650, which binds to Sheep IgG (H&L) in immunological assays.DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Sheep IgGDyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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