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Donkey anti-Sheep IgG (H&L), FITC conjugated

Catalog Number: GRP12398

Product Overview
Product Name Donkey anti-Sheep IgG (H&L), FITC conjugated
Catalog Number GRP12398
Species/Host Donkey
Product Properties
Form/Appearance Liquid
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Note For research use only.
Clonality Polyclonal
Purity Affinity purified IgG
Application Notes
Dilution Range 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)
Application Notes Additional Information: This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. Background: Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to sheep IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase sheep IgG.
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