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Donkey anti-Sheep IgG (H&L), ALP conjugated
Catalog Number: GRP12251
Product Overview
| Product Name | Donkey anti-Sheep IgG (H&L), ALP conjugated |
|---|---|
| Catalog Number | GRP12251 |
| Species/Host | Donkey |
| Reactivity | Sheep |
| Predicted Reactivity | Sheep IgG Heavy and Light chains (H&L) |
| Tested applications | ELISA, ICC, WB, IHC |
| Immunogen | Purified sheep IgG, whole molecule |
Product Properties
| Form/Appearance | Liquid |
|---|---|
| Storage | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Purified donkey IgG |
Application Notes
| Dilution Range | 1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA),1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) |
|---|---|
| Application Notes | Additional Information: No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. APL conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative Background: Donkey anti-sheep IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays. |
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