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Donkey anti-Sheep IgG (H&L), HRP conjugated
Catalog Number: GRP12235
Product Overview
| Product Name | Donkey anti-Sheep IgG (H&L), HRP conjugated |
|---|---|
| Catalog Number | GRP12235 |
| Species/Host | Donkey |
| Reactivity | Sheep |
| Predicted Reactivity | Sheep IgG Heavy and Light chains (H&L) |
| Tested applications | ELISA, WB, IHC |
| Immunogen | Purified sheep IgG, whole molecule |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Purified donkey IgG |
Application Notes
| Dilution Range | 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC) |
|---|---|
| Application Notes | Additional Information: No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatographyHRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, likedoneky serum or commercial formulations which are free from bovine IgG. Background: Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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