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Donkey anti-Rabbit IgG (H&L), ALP conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
Catalog Number: GRP12364
Product Overview
Product Name | Donkey anti-Rabbit IgG (H&L), ALP conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG |
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Catalog Number | GRP12364 |
Species/Host | Donkey |
Product Properties
Form/Appearance | Liquid |
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Storage | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
Note | For research use only. |
Clonality | Polyclonal |
Purity | Affinity purified IgG |
Application Notes
Dilution Range | 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC), 1 : 500-1 : 2000 (WB) |
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Application Notes | Additional Information: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG, based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative. Background: Donkey anti-rabbit IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to all rabbit IgGs in immunological assays. Antibody is adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG and affinity purified using solid phase rabbit IgG. |
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