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Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
Catalog Number: GRP12279
Product Overview
| Product Name | Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG |
|---|---|
| Catalog Number | GRP12279 |
| Species/Host | Donkey |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified IgG |
Application Notes
| Dilution Range | The optimal working dilution should be determined by the investigator. (ICC), immunohistochemistry ( IHC) (Flow cyt) |
|---|---|
| Application Notes | Additional Information: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative. Background: Donkey anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed to bovine,chicken,goat, guinea pig, hamster, horse,human,mouse,rat,sheep IgG and affinity purified using solid phase rabbit IgG. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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