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Donkey anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
Catalog Number: GRP12270
Product Overview
| Product Name | Donkey anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG |
|---|---|
| Catalog Number | GRP12270 |
| Species/Host | Donkey |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified IgG |
Application Notes
| Dilution Range | The optimal working dilution should be determined by the investigator. |
|---|---|
| Application Notes | Additional Information: This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase. Background: Donkey anti-rabbit IgG (H&L) is a secondary antibody, HRP conjugated, which binds to all rabbit IgGs in immunological assays. Antibody is specially adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG. Antibodies are affinity purified using solid phase rabbit IgG. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
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