Donkey anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse, rabbit, rat IgG

Catalog Number: GRP12314

Product Overview

Product Name	Donkey anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse, rabbit, rat IgG
Catalog Number	GRP12314
Species/Host	Donkey

Product Properties

Form/Appearance	Liquid
Storage	Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note	For research use only.
Clonality	Polyclonal
Purity	Affinity purified IgG

Application Notes

Dilution Range	The optimal working dilution should be determined by the investigator.
Application Notes	Additional Information: This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. Background: Donkey anti-goat IgG (H&L), HRP conjugated, is a secondary antibody which reacts with all goat IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.

Dilution Range

The optimal working dilution should be determined by the investigator.

Application Notes

Additional Information: This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. Background: Donkey anti-goat IgG (H&L), HRP conjugated, is a secondary antibody which reacts with all goat IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.

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