Der Bunte Tobias is a prestained protein ladder including a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recom-binant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. Der Bunte Tobias keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Reaction Setup
3 μl to 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Required materials but not provided
Vertical electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge
Guide for Molecular Weight Estimation (kDa)
Migration patterns of Der bunte Tobias Prestained Protein Ladder in different electrophoresis
conditions are listed below :
![Pattern]({{media url="wysiwyg/Guideformolecularweightestimation.jpg"}})
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel,respectively. 2.5 μl per well for general Western transferring.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
