Availability

Der bunte Tobias - prestained protein ladder (6,5 - 270 Kda)

Catalog Number: GRP13284

Product Overview
Product Name Der bunte Tobias - prestained protein ladder (6,5 - 270 Kda)
Catalog Number GRP13284
Tested applications WB
Product Properties
Form/Appearance Ready to use
Preservatives Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol
Storage Stable up to 2 weeks at 25°C ,Stable up to 3 months at 4°C, Stable up to 24 months at -20°C
Note For research use only.
Product Description

Der Bunte Tobias is a prestained protein ladder including a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recom-binant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. Der Bunte Tobias keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Reaction Setup

3 μl to 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Required materials but not provided

Vertical electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge

Guide for Molecular Weight Estimation (kDa)

Migration patterns of Der bunte Tobias Prestained Protein Ladder in different electrophoresis

conditions are listed below :

Pattern

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel,respectively. 2.5 μl per well for general Western transferring.


Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Validation Images
Reviews
Write Your Own Review
You're reviewing:Der bunte Tobias - prestained protein ladder (6,5 - 270 Kda) - GRP13284
Your Rating