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Collagen III antibody [C2C3-2], C-term

Catalog Number: GRP49

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Datasheet
Product Overview
Product Name Collagen III antibody [C2C3-2], C-term
Catalog Number GRP49
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, WB
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III. The exact sequence is proprietary.
Alternative Names (click to expand) collagen type III alpha 1 chain | EDS4A | EDSVASC
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.67 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P02461
Entrez 1281
Product Description

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000
Validation Images
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III alpha1 antibody [C2C3-2], C-term (GRP501) diluted at 1:1000.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III alpha1 antibody [C2C3-2], C-term (GRP501) diluted at 1:1000.
Collagen III antibody [C2C3-2], C-term detects Collagen III protein at secreted on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Collagen III antibody [C2C3-2], C-term (GRP501) dilution: 1:500.
Collagen III antibody [C2C3-2], C-term detects Collagen III protein at secreted on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Collagen III antibody [C2C3-2], C-term (GRP501) dilution: 1:500.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Collagen III (GRP501) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Collagen III (GRP501) antibody at 1:500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using Collagen III(GRP501) antibody at 1:50 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using Collagen III(GRP501) antibody at 1:50 dilution.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3-2], C-term (GRP501) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3-2], C-term (GRP501) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
The ICC/IF analysis of Collagen III antibody [C2C3-2], C-term was published by Saito Y and colleagues in the journal Sci Rep in 2017 .
The ICC/IF analysis of Collagen III antibody [C2C3-2], C-term was published by Saito Y and colleagues in the journal Sci Rep in 2017 .
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