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Product Overview
| Product Name | cIAP1 antibody |
|---|---|
| Catalog Number | GRP44 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q13490 |
| Entrez | 329 |
Product Description
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
cIAP1 antibody detects cIAP1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. cIAP1 antibody (GRP496) diluted at 1:500.
Sample (30 μg of whole cell lysate) A: JurKat 7.5% SDS PAGE GRP496 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sample (50 μg of whole cell lysate) A: Rat muscle 7.5% SDS PAGE GRP496 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody (GRP496) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the
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