Choline Acetyltransferase antibody [N1N3]

Catalog Number: GRP134

Product Overview

Product Name	Choline Acetyltransferase antibody [N1N3]
Catalog Number	GRP134
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-Fr, IHC-P, WB
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of human Choline Acetyltransferase. The exact sequence is proprietary.
Alternative Names	(click to expand) choline O-acetyltransferase \| CHOACTASE \| CMS1A \| CMS1A2 \| CMS6

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	1.26 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P28329
Entrez	1103

Product Description

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq]

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000

Validation Images

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein in the amacrine cells by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Choline Acetyltransferase protein stained by Choline Acetyltransf

Non-transfected (â€“) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GRP586) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antib

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GRP586) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GRP586) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GRP586) di

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GRP586

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse spinal cord.Red: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GRP586) dil

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Choline Acetyltransferase protein st

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