Chicken anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse,rabbit IgG/serum

Catalog Number: GRP12449

Product Overview

Product Name	Chicken anti-Goat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse,rabbit IgG/serum
Catalog Number	GRP12449
Species/Host	Chicken

Product Properties

Form/Appearance	Lyophilized
Storage	Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note	For research use only.
Clonality	Polyclonal
Purity	Affinity purified IgY

Application Notes

Dilution Range	1 : 10 000-1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
Application Notes	Additional Information: This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins and human,mouse,rabbit IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. Background: Chicken anti-goat IgG (H&L), HRP conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are adsorbed against to human,mouse,rabbit IgG/serum and affinity purified using solid phase goat IgG. Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Dilution Range

1 : 10 000-1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Application Notes

Additional Information: This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins and human,mouse,rabbit IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. Background: Chicken anti-goat IgG (H&L), HRP conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are adsorbed against to human,mouse,rabbit IgG/serum and affinity purified using solid phase goat IgG. Reconstitution: For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

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