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CD44 antibody

Catalog Number: GRP25

Product Overview
Product Name CD44 antibody
Catalog Number GRP25
Species/Host Rabbit
Reactivity Human, Mouse, Rat, Rabbit
Conjugation Unconjugated
Tested applications ICC, IF, IHC-Fr, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human CD44. The exact sequence is proprietary.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 0.64 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P16070
Entrez 960
Product Description

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:1000-1:20000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000
Validation Images
CD44 antibody detects CD44 protein at membrane and cytoplasm on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma . CD44 antibody (GRP477) diluted at 1:500.
Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GRP477) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the prim
Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CD44(GRP477) antibody at 1:100 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44(GRP477) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GRP477) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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