CBS antibody

Catalog Number: GRP139

Product Overview

Product Name	CBS antibody
Catalog Number	GRP139
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.
Alternative Names	(click to expand) cystathionine-beta-synthase \| CBSL \| HIP4

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P35520\|P0DN79
Entrez	875

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500

Validation Images

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS(GRP591) antibody at 1:500 dilution.

CBS antibody detects CBS protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: CBS protein stained by CBS antibody (GRP591) diluted at 1:200.Blue: Hoechst 33342 staining.

CBS antibody detects CBS protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GRP591) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to

Non-transfected (â€“) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GRP591) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the p

CBS antibody detects CBS protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GRP591) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was use

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GRP591) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

CBS antibody immunoprecipitates CBS protein in IP experiments.IP samples: HepG2 whole cell extractA. 50 ?g HepG2 whole cell extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of CBS protein by 4 ?g CBS antibody (GRP591)7.5 % SDS-PAG

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