AKR1C1 antibody

Catalog Number: GRP39

Product Overview

Product Name	AKR1C1 antibody
Catalog Number	GRP39
Species/Host	Rabbit
Reactivity	Human, Mouse
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-P, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.
Alternative Names	(click to expand) aldo-keto reductase family 1 member C1 \| 2-ALPHA-HSD \| 20-ALPHA-HSD \| C9 \| DD1 \| DD1/DD2 \| DDH \| DDH1 \| H-37 \| HAKRC \| HBAB \| MBAB

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	1.02 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	Q04828
Entrez	1645

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000

Validation Images

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GRP491 diluted at 1:1000

AKR1C1 antibody detects AKR1C1 protein by western blot analysis.A. 50 μg mouse liver lysate/extract10% SDS-PAGEAKR1C1 antibody (GRP491) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GRP491) diluted at 1:1000.

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(GRP491) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(GRP491) antibody at 1:500 dilution.

Non-transfected (â€“) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GRP491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the

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