Availability

Der Blaue Jonas - Single-step Coomassie Blue protein gel dye - HS Code 38220000 - Diagnostikreagenzien, Laborreagenzien

Catalog Number: GRP1

Product Overview
Product Name Der Blaue Jonas - Single-step Coomassie Blue protein gel dye - HS Code 38220000 - Diagnostikreagenzien, Laborreagenzien
Catalog Number GRP1
Product Properties
Storage 4 degrees Celsius. Shipment at RT.
Product Description

1 x 1000 ml bottle

Product Code GRP1

 

Description

Der Blaue Jonas is a one-step protein gel dye that requires no pre- or post- gel treatment. Just run your protein gel, add the stain and watch your bands appear.

 

Storage                      Shelf Life

4°C                              1 year

 

Protocoll

Add enough stain to cover gel completely. Incubate at room temperature according to the times listed below for lowest detectable level in a protein band. Gently agitate if possible.

1) 1 ug or more 2 minutes

2) 100 ng to 1 ug 10 minutes

3) 10ng to 100ng 60 minutes

It is possible to decrease these times by inserting gel and stain into microwave for 20 seconds.

For maximum sensitivity follow staining by a 20 minute gentle agitation in dionized water.

 

Protocoll for Mass Spectrometry

1) Cut out protein band and place in microfuge tube

2) Add 1 ml 30% ethanol or 30% acetone (30% acetic acid may be used but will result in acetylation of N-terminus).

3) Incubate for 30 minutes.

4) Repeat steps 2 and 3 until all stain removed from protein (typically 2 or 3 washes are required for complete stain removal)

5) Process as typical for mass spec analysis.

Application Notes
Application Notes for up to 150 mini-gels (ready to use)
Validation Images
Decreasing amounts of BSA were run on a 10% SDS-PAGE gel and stained with Der Blaue Jonas for 10 minutes. The arrow denotes the 100 ng BSA lane.
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