Goat anti-Chicken IgY (H&L), HRP conjugated

Product Overview

Product Name	Goat anti-Chicken IgY (H&L), HRP conjugated
Catalog Number	GRP12234
Species/Host	Goat
Reactivity	Chicken
Tested applications	ELISA, WB, IHC
Immunogen	Purified chicken IgY, whole molecule.

Product Properties

Form/Appearance	Lyophilized
Storage	Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Note	For research use only.
Clonality	Polyclonal
Purity	Affinity purified goat IgG, using solid phase chicken IgY.

Application Notes

Dilution Range	1 : 10 000 -1 : 150 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 20 000 and 1 : 10 000 (WB)
Application Notes	Additional Information: Antibody binds to:heavy chains on chicken IgYlight chains on all chicken immunoglobulinsNo reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY. This HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Background: Goat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays. Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

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