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Product Overview
| Product Name | GP - Glycogen phosphorylase |
|---|---|
| Catalog Number | GRP12223 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Rabbit |
| Predicted Reactivity | Atlantic Salmon, Bovine, Dog, Drosophila melanogaster, Hen, Horse, Pig, Zebrafish, Xenopus laevis |
| Tested applications | IP, WB |
| Immunogen | KLH-conjugated peptide derived from the sequence of human glycogen phosphorylase P11217 |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Purity | Affinity purified serum |
| MW | 97 kDa |
| Uniprot ID | P11217 |
Application Notes
| Dilution Range | 5 µg (IP), 1 : 2000 (WB) |
|---|---|
| Application Notes | Additional Information: Samples used to test this antibody were: muscle and liver homogenates, purified glycogen. This antiboy will recogize glycogen phosphorylase from both liver and muscle. It has been used in ICC on human primary myotubes. This antibody can detect GP in crude tissue homogenate in both, liver and muscle tissue and is able to immunoprecipiate GP.Glycogen phosphorylase is a good marker for determining the degree of postmortem protein denaturation in muscle. When glycogen phosphorylase denatures it shifts from the sarcoplasmic fraction of the muscle to the myofibrillar fraction of the muscle. This shift is detectable using SDS-PAGE and western blotting techniques. The antibody is being used to detect glycogen phosphorylase levels in both sarcoplasmic and myofibrillar protein extracts from chicken muscle. Background: Glycogen phosphorylase (EC 2.4.1.1) is an enzyme which is catalyzing the rate limiting step in the degradation of glycogen in animals by releasing glucose-1-phosphate from the terminal alpha1,4-glycosidic bond. Reconstitution: For reconstitution add 50 µl of sterile water |
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