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Product Overview
| Product Name | TRPM2 antibody [N1N2-2], N-term |
|---|---|
| Catalog Number | GRP131 |
| Species/Host | Rabbit |
| Reactivity | Human, Rat |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human TRPM2. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
|---|---|
| Concentration | 1.38 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | O94759 |
| Entrez | 7226 |
Product Description
The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq]
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000 |
|---|
Validation Images
![TRPM2 antibody [N1N2-2], N-term detects TRPM2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. TRPM2 antibody [N1N2-2], N-term (GRP583) diluted at 1:500.](https://www.grp-ak.de/media/catalog/product/t/r/trpm2-antibody-n1n2-2-n-term_grp583_ihc-p_1_2.jpg)
![HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TRPM2 antibody [N1N2-2], N-term (GRP583) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antib](https://www.grp-ak.de/media/catalog/product/t/r/trpm2-antibody-n1n2-2-n-term_grp583_wb_1_2.jpg)
Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using TRPM2(GRP583) antibody at 1:100 dilution.
TRPM2 antibody [N1N2-2], N-term detects TRPM2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. TRPM2 antibody [N1N2-2], N-term (GRP583) diluted at 1:500.
HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TRPM2 antibody [N1N2-2], N-term (GRP583) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antib
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