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Product Overview
        
                           
                                                
                                                        
                                    
    
        | Product Name | Tyrosine Hydroxylase antibody [N1C1] | 
|---|---|
| Catalog Number | GRP110 | 
| Species/Host | Rabbit | 
| Reactivity | Human, Mouse, Rat, Zebrafish | 
| Conjugation | Unconjugated | 
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB | 
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary. | 
| Alternative Names | (click to expand) | 
Product Properties
        
                           
                                                
                                                        
                                    
    
        | Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. | 
|---|---|
| Concentration | 0.8 mg/ml | 
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. | 
| Note | For research use only. | 
| Isotype | IgG | 
| Clonality | Polyclonal | 
| Purity | Purified by antigen-affinity chromatography. | 
| Uniprot ID | P07101 | 
| Entrez | 7054 | 
Product Description
        
                           
                             
                    
    
        The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]
Application Notes
        
                           
                                                
                                                        
                                    
    
        | Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,IHC-Wm: 1:100-1:500 | 
|---|
Validation Images
        
                                          
                    
                        
                                                            
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                    
                                    
                                                                
                                                        
                    
                
               
                    
    
        
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) di                                    
                                    
                                
                                        Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody  was used to detect the primary antibody.                                    
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Tyrosine Hydroxylase antibody [N1C1] (GRP562) dilution: 1:100.                                    
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (G                                    
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Tyrosine Hydroxylase antibody [N1C1] (GRP562) dilution: 1:100.                                    
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) di                                    
                                    
                                
                                        Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody  was used to detect the primary antibody.                                    
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at 1:1000.An                                    
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at 1:1000.Anti                                    
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at                                     
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase                                     
                                    
                                
                                        Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) dil                                    
                                    
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![Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody  was used to detect the primary antibody.](https://www.grp-ak.de/media/catalog/product/t/y/tyrosine-hydroxylase-antibody-n1c1_grp562_wb_1_2.jpg) 
                                        ![Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at 1:1000.An](https://www.grp-ak.de/media/catalog/product/t/y/tyrosine-hydroxylase-antibody-n1c1_grp562_ihc-p_2_2.jpg) 
                                        ![Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at 1:1000.Anti](https://www.grp-ak.de/media/catalog/product/t/y/tyrosine-hydroxylase-antibody-n1c1_grp562_ihc-p_1_2.jpg) 
                                        ![Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) diluted at](https://www.grp-ak.de/media/catalog/product/t/y/tyrosine-hydroxylase-antibody-n1c1_grp562_ihc_4_2.jpg) 
                                        ![Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase](https://www.grp-ak.de/media/catalog/product/t/y/tyrosine-hydroxylase-antibody-n1c1_grp562_if_2_2.jpg) 
                                        ![Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GRP562) dil](https://www.grp-ak.de/media/catalog/product/t/y/tyrosine-hydroxylase-antibody-n1c1_grp562_ihc_2_2.jpg) 
                                        ![Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxyla](https://www.grp-ak.de/media/catalog/product/t/y/tyrosine-hydroxylase-antibody-n1c1_grp562_if_1_2.jpg) 
                                        