PAX6 antibody

Catalog Number: GRP137

Product Overview

Product Name	PAX6 antibody
Catalog Number	GRP137
Species/Host	Rabbit
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-Fr, IHC-P, IP, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human PAX6. The exact sequence is proprietary.
Alternative Names	(click to expand) paired box 6 \| AN \| AN2 \| ASGD5 \| D11S812E \| FVH1 \| MGDA \| WAGR

Product Properties

Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	0.85 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG
Clonality	Polyclonal
Purity	Purified by antigen-affinity chromatography.
Uniprot ID	P26367
Entrez	5080

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,IP: 1:1000-1:1000

Validation Images

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GRP589) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (â€“) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GRP589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the p

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GRP589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PAX6 protein stained by PAX6 antibody (GRP589) diluted at 1:500.Red: Phalloidin, a cytoskeleton mar

PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody (GRP589) diluted at 1:250.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GRP589) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PAX6 antibody immunoprecipitates PAX6 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 ?g 293T whole cell lysate/extract B. Control with 2 ?g of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 ?g of P

PAX6 antibody detects PAX6 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody (GRP589) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by be

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Samples: Paraffin-Embedded mouse retina.Green: PAX6 protein stained by PAX6 antibody (GRP589) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338]

PAX6 antibody detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: PAX6 stained by PAX6 antibody (GRP589) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tub

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