Availability

Anti-HLA-G Purified

Catalog Number: GRP10596

Product Overview
Product Name Anti-HLA-G Purified
Catalog Number GRP10596
Reactivity Human
Tested applications ELISA, FC, ICC, IHC-Fr, IHC-P, WB
Immunogen C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
Product Properties
Concentration 1 mg/ml
Storage Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Note For research use only.
Isotype Mouse IgG1
Clonality Monoclonal
Clone ID 5A6G7
Uniprot ID P17693
NCBI 3135
Product Description

The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.

Application Notes
Application Notes Immunohistochemistry (frozen sections): recommended dilution: 10 μg/ml, positive tissue: placenta
Flow cytometry: recommended dilution: 2 μg/ml
Immunohistochemistry (paraffin sections): recommended dilution: 10 μg/ml, positive tissue: placenta
ELISA: positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant, negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100).
Western blotting: positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE
Reviews
Write Your Own Review
You're reviewing:Anti-HLA-G Purified - GRP10596
Your Rating