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Product Overview
| Product Name | DLK/MAP3K12 antibody |
|---|---|
| Catalog Number | GRP153 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | IHC-Fr, IHC-P, WB |
| Immunogen | Recombinant protein encompassing a sequence within the center region of mouse DLK/MAP3K12. The exact sequence is proprietary. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
|---|---|
| Concentration | 1 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | Q60700 |
| Entrez | 26404 |
Product Description
May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
Application Notes
| Dilution Range | WB: 1:500-1:3000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000 |
|---|
Validation Images
DLK/MAP3K12 antibody detects DLK/MAP3K12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GRP605) diluted by 1:1000. The HRP-conjugated anti-ra
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAP3K12(GRP605) antibody at 1:250 dilution.
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GRP605) diluted at 1:1000.
Sample (50 μg of whole cell lysate) A: mouse brain 7.5% SDS PAGE GRP605 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
DLK/MAP3K12 antibody detects MAP3K12 protein by Western blot analysis.A. 20 ?g mouse embryonic stem cell whole cell lysate/extract 7.5 % SDS-PAGEDLK/MAP3K12 antibody (GRP605) dilution: 1:1000
DLK/MAP3K12 antibody detects DLK/MAP3K12 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody (GRP605) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature
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