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Product Overview
| Product Name | Anti-HLA-G Purified |
|---|---|
| Catalog Number | GRP10596 |
| Reactivity | Human |
| Tested applications | ELISA, FC, ICC, IHC-Fr, IHC-P, WB |
| Immunogen | C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin. |
Product Properties
| Concentration | 1 mg/ml |
|---|---|
| Storage | Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. |
| Note | For research use only. |
| Isotype | Mouse IgG1 |
| Clonality | Monoclonal |
| Clone ID | 5A6G7 |
| Uniprot ID | P17693 |
| NCBI | 3135 |
Product Description
The mouse monoclonal antibody 5A6G7 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Application Notes
| Application Notes | Immunohistochemistry (frozen sections): recommended dilution: 10 μg/ml, positive tissue: placenta Flow cytometry: recommended dilution: 2 μg/ml Immunohistochemistry (paraffin sections): recommended dilution: 10 μg/ml, positive tissue: placenta ELISA: positive control: HeLa/HLA-G5 transfectants cell lysate, HeLa/HLA-G5 cell supernatant, negative control: HeLa cell lysate. The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody W6/32 (cat. no. 1B-422-C100). Western blotting: positive control: JEG-3 cell lysate, reducing conditions, 12% AA SDS-PAGE |
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