Availability

  • Request Lead Time
  • In stock and ready for quick dispatch
  • Usually dispatched within 5-10 working days

S100 beta antibody

Catalog Number: GRP158

Product Overview
Product Name S100 beta antibody
Catalog Number GRP158
Species/Host Rabbit
Reactivity Human, Mouse, Rat, Squirrel
Conjugation Unconjugated
Tested applications ICC, IF, IHC-Fr, IHC-P, WB
Immunogen Full length human S100 beta Recombinant protein.
Alternative Names
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 1.05 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID P04271
Entrez 6285
Product Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Application Notes
Dilution Range WB: 1:500-1:3000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000
Validation Images
S100B antibody detects S100B protein at glial cell on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. S100B antibody (GRP610) dilution: 1:500.
Whole cell extract (30 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with S100B antibody (GRP610) diluted at 1:500.
S100B antibody detects S100B protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g whole cell lysate/extract of V5-human S100B-transfected 293T cells15 % SDS-PAGES100B antibody (GRP610) dilution: 1:1000
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody (GRP610) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Mouse tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody (GRP610) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
S100 beta antibody detects S100 beta protein expression by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: S100 beta stained by S100 beta antibody (GRP610) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubuli
S100 beta antibody detects S100 beta protein at glia cells by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: S100 beta stained by S100 beta antibody (GRP610
Reviews
Write Your Own Review
You're reviewing:S100 beta antibody - GRP158
Your Rating