Rad50 antibody [13B3]

Product Overview

Product Name	Rad50 antibody [13B3]
Catalog Number	GRP89
Species/Host	Mouse
Reactivity	Human, Mouse, Rat, Monkey
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-P, IP, WB
Immunogen	Amino acids 1-425 of Rad50 expressed in E. coli.
Alternative Names	(click to expand) Rad50 Double Strand Break Repair Protein \| Nbsld \| Rad502 \| Hrad50 \| Rad50

Product Properties

Form/Appearance	Liquid: PBS
Concentration	1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG1
Clonality	Monoclonal
Purity	Affinity purified by Protein G.
Clone ID	13B3
Uniprot ID	Q92878
Entrez	10111

Product Description

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000

Validation Images

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad50 protein stained by Rad50 antibody [13B3] (GRP541) diluted at 1:200.Red: phalloidin, a c

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GRP541) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. Rad50 antibody [13B3] (GRP541) diluted at 1:200.

Rad50 antibody [13B3] detects Rad50 protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. Rad50 antibody [13B3] (GRP541) diluted at 1:200.

Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC-3 xenograft. Rad50 antibody [13B3] (GRP541) diluted at 1:200.

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GRP541) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The WB analysis of Rad50 antibody [13B3] was published by Palagyi A and colleagues in the journal Mol Cancer in 2010 .

The WB, IP analysis of Rad50 antibody [13B3] was published by MariggiÃ² G and colleagues in the journal PLoS Pathog in 2017.PMID: 28430817

The WB analysis of Rad50 antibody [13B3] was published by MariggiÃ² G and colleagues in the journal PLoS Pathog in 2017.PMID: 28430817

The WB analysis of Rad50 antibody [13B3] was published by Zhu J and colleagues in the journal EMBO Mol Med in 2013.PMID: 23341130

The WB analysis of Rad50 antibody [13B3] was published by Harten SK and colleagues in the journal BMC Biol in 2015.PMID: 25857663

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Rad50 antibody [13B3]

Catalog Number: GRP89