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Rad51 antibody [N1C2]

Catalog Number: GRP8

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Datasheet
Product Overview
Product Name Rad51 antibody [N1C2]
Catalog Number GRP8
Species/Host Rabbit
Reactivity Human, Mouse, Rat, Zebrafish
Conjugation Unconjugated
Tested applications ICC, IF, IHC-P, IP, WB
Immunogen Recombinant protein encompassing a sequence within the center region of human Rad51. The exact sequence is proprietary.
Alternative Names (click to expand) RAD51 recombinase | BRCC5 | FANCR | HRAD51 | HsRad51 | HsT16930 | MRMV2 | RAD51A | RECA
Product Properties
Form/Appearance Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration 1.53 mg/ml
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by antigen-affinity chromatography.
Uniprot ID Q06609
Entrez 5888
Product Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. [provided by RefSeq]

Application Notes
Dilution Range WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IP: 1:100-1:500
Validation Images
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was
Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad51 protein stained by Rad51 antibody [N1C2] (GRP460) diluted at 1:500.Red: phalloidin, a c
Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rad51 protein stained by Rad51 antibody [N1C2] (GRP460) diluted at 1:500.Red: phalloidin, a c
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GRP460) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GRP460) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GRP460) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GRP460) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Rad51 antibody [N1C2] detects Rad51 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human cervical carcinoma.Rad51 stained by Rad51 antibody [N1C2] (GRP460) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH
Rad51 antibody [N1C2] detects Rad51 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human cervical carcinoma.Rad51 stained by Rad51 antibody [N1C2] (GRP460) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH
Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Rad51 stained by Rad51 antibody [N1C2] (GRP460) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Rad51 stained by Rad51 antibody [N1C2] (GRP460) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Rad51 stained by Rad51 antibody [N1C2] (GRP460) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rad51 antibody [N1C2] detects Rad51 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Rad51 stained by Rad51 antibody [N1C2] (GRP460) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pri
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pri
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [N1C2] (GRP460) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was
Rad51 antibody [N1C2] immunoprecipitates Rad51 protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 ?g Jurkat whole cell extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of Rad51 protein by 4 ?g Rad51 antibody [N1C
Rad51 antibody [N1C2] immunoprecipitates Rad51 protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 ?g Jurkat whole cell extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of Rad51 protein by 4 ?g Rad51 antibody [N1C
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